Why can frequent douching be a predisposing factor to bacterial vaginosis, Vulvovaginal candidiasis or trichomoniasis?

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Answer Critical Thinking Questions 1-4 for Chapter 26 on page 771. Answers should be submitted in a word document with any associated references used.
1) The tropical skin disease called yams is transmitted by direct contact. It causative agent, Treponema pallidum pertenue , is indistinguishable from T. Pallidum. Syphilis epidemics in Europe coincided with the return of Columbus from the New World. How might T. Pallidum pertenue have evolved into T. Pallidum in the temperature climate of Europe?
2) Why can frequent douching be a predisposing factor to bacterial vaginosis, Vulvovaginal candidiasis or trichomoniasis?
3) Neisseria is cultured on Thayer-Martin media consisting of chocolate agar and nystatin incubated in a 5% CO2 environment. How is this selective for Neisseria?
4) The list below is a key selected microorganism that cause genitourinary infections. Complete this by listing genera discussed in this chapter in the blanks that correspond to their respective characteristics
Gram-negative bacterial Spirochete Aerobic
Anaerobic Coccus
Oxidase positive
Bacillus nonmotile
Gram positive wall
Obligate intracellular parasite
Lacking cell wall
Urease-negative
Fungus
Pseudohypae Protozoa
Flagella
No organism observed/cultured from patient

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