PU Trigeminal Neuralgia Poster Presentation and Script

Poster Design Fundamentals: • A1 portrait / landscape • Clear, well organised layout • Simple but effective design scheme • Good font size • Attractive, high-resolution figures Header ~ 10% of the poster: • • • • Title Name Institution and address (Dept., Institution, City, Country) Logo (Institutions, potentially any funding body) What makes a good scientific title? • Likely to be found on a search • Clearly explains what the work is • Hits lots of keywords Sections Research Poster • • • • • (Abstract) Introduction Materials & Methods Results Conclusions Review Poster • • • • (Abstract) Introduction Discussion Conclusions • References • References Introduction Think about appropriate focus: • Your poster should concentrate on therapies; i.e. the discussion • Your introduction should therefore concentrate on the basic science to provide a background for the therapy • Therefore very, very briefly cover things like the symptoms, potentially ignore epidemiology etc. Organisational flow TITLE Left → Right Top → bottom (e.g. book) You can do something different, but it’s up to you to make it clear Top → bottom Left → Right (e.g. newspaper columns) TITLE Basic sample poster designs TITLE TITLE Intro Discussion Intro Figure 1 Disc. Fig 1 Disc. Fig 2 Figure 2 Boom! Fig 3 Conc. Conclusions Figure 3 Refs Refs Synchronous neuronal responses evoked by astrocytic glutamate release • Clear and informational title R. E. Sims, G. Saunders, H. R. Parri School of Life and Health Sciences, Aston University, Birmingham, U.K. Materials and methods Introduction Control n=8, enhanced n=6 20 Control Enhanced release * 15 15 SCRs / min * 10 SCRs / min 15 10 ** 10 5 5 5 0 0 0 b +AP5 CTX (n=6) 0.6 CTX Figure 1: Enhanced astrocytic release protocol increases the rate of spontaneous Ca2+ responses. SCRs were recorded from neurones located in 440 x 340 μm areas of cortex, CA1 and ventrobasal thalamus with TTX (consistent with non-synaptic release) in control or enhanced astrocyte release conditions. VB Control n=7, enhanced n=7 20 * 0.4 0.2 0.0 CA1 TTX 0.8 AP5 0.6 CA1 (n=7) ** 0.4 0.2 0.0 TTX AP5 0.2 TTX AP5 Figure 2: Gliotransmitter-derived neuronal Ca2+ responses are primarily mediated by NMDA receptors. SCRs in enhanced release conditions were recorded in cells from cortex, CA1 hippocampus and ventrobasal thalamus. Raw data traces from single experiments show normalised fluorescence from neurones in the presence of 1μM TTX and and TTX + 100μM AP5 in each brain area (a). The addition of AP5 significantly reduced the number of SCRs in each brain area (b). VB • Clean layout – clearly defined and separated sections ** 0.4 0.0 • Simple colour scheme VB (n=6) 0.6 SCRs cell-1 min-1 CA1 Control n=11, enhanced n= 6 SCRs cell-1 min-1 TTX CTX 20 SCRs cell-1 min-1 a Cortical, hippocampal and thalamic slices were obtained from 7-14-day-old Wistar rats in accordance with Home Office guidelines. Slices were incubated in artificial cerebrospinal fluid (aCSF) with an enhanced astrocytic release protocol for at least two hours [1]. aCSF was composed of (in mM): NaCl, 126; KCl, 2.5; NaHCO3, 26; KH2PO4, 1.25; CaCl2, 2, MgSO4; equilibrated with 95%:5% O2:CO2. Slices were then loaded with cell-permeable Fluo-4 and experiments conducted in Mg2+-free aCSF and TTX. Data is presented as mean ± s.e.m. Statistical sign