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Expressing a gene from one organism in the cell of another species has many challenges and obstacles, especially since post-translational modification of proteins is so different among organisms. For example, when human recombinant insulin was first expressed in E. coli many strategies had to be devised to achieve proper folding and disulfide bond formation in the insulin. Give two specific examples of a post-translational modifications important is forming a functional protein. For each, suggest a way to recover functionality when making a protein in a non-native organism.
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