Calculate the concentration of an unknown DNA sample. The total volume of the sample is 560 μL. A 25 μL aliquot, diluted to 1 mL, gives an A260 reading of 0.25. An A260 of 1.0 is equivalent to a DNA concentration of 50 μg/mL.

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Calculate the concentration of an unknown DNA sample. The total volume of the sample is 560 μL. A 25 μL aliquot, diluted to 1 mL, gives an A260 reading of 0.25. An A260 of 1.0 is equivalent to a DNA concentration of 50 μg/mL.

 

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